Coding

Part:BBa_K4805010:Design

Designed by: Yuhan Gan   Group: iGEM23_LINKS-China   (2023-10-12)


pTEF2-ERG20_F96W-tSSA1-pPGK1-ClSS-tPGK1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1308
    Illegal BglII site found at 1369
    Illegal BglII site found at 2850
    Illegal BglII site found at 3945
    Illegal XhoI site found at 1493
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1024
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1479
    Illegal BsaI site found at 1748
    Illegal BsaI.rc site found at 1729
    Illegal BsaI.rc site found at 2860


Design Notes

Its DNA sequences have been optimized which can be expressed in S. cerevisiae.



Source

DNA synthesis

References